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CRM / Certified Reference Materials
 National Institute for Minamata Disease (NIMD) has produced certified reference materials, CRM (hair and urine), which are based on a quality control system that conforms to ISO Guide 35. You can find these items on the RMinfo (Reference Materials Total Information Services of Japan) and COMAR (Committee on Man and Radiation) data base.
Distribution target and condition
 NIMD CRM will be handed out for free to the laboratories in universities, public institutes, and private research institutes that perform chemical analysis related to the Minamata Convention on Mercury. Please answer questions below when applying. Your feedback will enable us to improve our new product. Transfer and sale will be prohibited in principle.
Information on distribution
 Please check the distribution conditions above.
If you would like to apply, please send an E-mail to the following address with the required information below.
rminfo@env.go.jp
(contact person : HARAGUCHI Koichi)
※Please copy and paste the following details info the text and answer all questions
①Applicant (full name):
②Name of institute:
③Dept./Section:
④Address:
 City
 State/Province/Region
 Zip/Postal Code
 Country
⑤Phone number:
⑥E-Mail:
⑦Purpose of use
 A: Method development
 B: Exposure level studies
 C: Metabolic studies
 D: Environmental studies
 E: Others ( )
⑧Which certified or reference values do you need?
 A: MeHg
 B: THg
 C: Cu
 D: Zn
 E: As
 F: Se
 G: Cd
⑨Does the concentration meet your needs?
 A: High
 B: Just right
 C: Low
 D: N/A
⑩Witch type of quality control materials do you use in routine analysis ( If you have any other request)?
 A: Hair
 B: Urine
 C: Blood
 D: Fish and mussels
 E: Sediments
 F: Soils
 G: Waters
 H: Spiked Samples
 I: Others ( )
⑪Which key properties of reference material are important when selecting a CRM?
 A: Homogeneity in small amount
 B: Stability at room temperature
 C: Concentration level
Please answer ⑫ only when you apply for NIMD-01.
⑫Does the minimum requirement (quantity) meet your needs?
 A: Too much
 B: Just right
 C: Too little
 D: N/A
 The item will be shipped within a month.
Please note that shipping is not guaranteed if the distribution conditions are not met or if any questions are unanswered.
「NIMD-01 Human Hair」
1. Certified Value and Indicative Value
 Certified values of NIMD-01 shown below are traceable to the International System of Units (SI). Expanded uncertainties of certified values are obtained by multiplying standard uncertainties by a coverage factor k = 2.
Class Elements Valu
(mg/kg)
Expanded Uncertainty
(mg/kg)
Analytical Method
(Refer to annotations below)
Certified
Value
Methyl Mercury (Me-Hg) 0.634 0.071 1),2),3)
Total Mercury (THg) 0.794 0.050 4),5),6)
Copper (Cu) 12.8 1.4 7)
Zinc (Zn) 234 29 7)
Selenium (Se) 1.52 0.29 7)
Indicative
Value
Arsenic (As) 0.17 0.03
Analytical method
1) GC-electron capture detector  5) Cold vapor atomic absorption
2) HPLC-chemiluminescence 6) Cold vapor atomic fluorescence
3) Ethylation-GC-cold vapor atomic fluorescence 7) Inductively coupled plasma mass spectrometry
4) Thermal decomposition atomic absorption  
2.Minimum Sampling Volume
 Sampling volume for an analysis should be 20 mg or more to ensure the homogeneity.
3.Period of Validity
 The period of validity of NIMD-01 is Oct.01 2021 if stored unopened and under the condition satisfying the instructions on storage shown below. The period of validity can be prolonged when further stability was ensured. Updated information on period of validity will be announced here.
4.Product Form
 NIMD-01 is provided in gray powder form and put in a glass bottle. Each bottle contains approximately 3 g of the reference material.
5.Instruction on Storage
 Store in a clean place and avoid light and high temperature (less than 35℃, frozen/refrigerated storage recommended).
6.Instruction for Handle and Use
 (1)Although NIMD-01 is sterilized with γ-ray irradiation, the potential risk as a source of diseases should be considered
 (2)To homogenize the contents, shake the bottle well before use.
 (3)Leaved the bottle at room temperature for more than 30 minutes before measuring sample mass for analytical use.
    Sampling volume for an analysis should be 20 mg or more to ensure the homogeneity.
 (4)Moisture rate of NIMD-01 is approximately 5%. All of the certified values were calculated with dry mass.
    Therefore, measure moisture content of samples to correct obtained values. To measure moisture content, follow
    the instructions.
    Measure out samples ( >100 mg) in weighing bottles and leave them in an oven at 85℃ for 4 hours. After heating,
    close the lid immediately and let them cool in a desiccator for 30 minutes. Measure their mass and compare with
    those measured before heating. The weight difference can be calculated as a moisture content.
    To avoid evaporation and degradation of components, samples for metrological analysis and those for moisture
    content measurement should be prepared separately.
7.Participants Involved in Characterization of NIMD-01
No. Laboratory Country
1 Balai Besar Laboratorium Kesehatan (BBLK) Indonesia
2 Dong-A University Korea
3 IDEA Consultants, Inc. Japan
4 Institut national de santé publique du Québec Canada
5 International Mercury Lab. Japan
6 Jožef Stefan Institute Slovenia
7 Kagoshima University Japan
8 Lumex Instruments Russia
9 Milestone General.K.K Japan
10 National Institute for Environmental Studies (NIES) Japan
11 National Institute for Minamata Disease (NIMD) Japan
12 Nippon Instruments Corporation (NIC) Japan
13 Pusat Penelitian Dan Pegembangan Kualitas Dan Laboratorium Lingkungan (P3KLL) Indonesia
14 SHIMADZU Techno-Research, Inc. Japan
15 St. Petersburg University Russia
16 Tshwane University of Technology South Africa
17 University of Ottawa Canada
18 University of Rochester School of Medicine and Dentistry US
19 University of Southern Denmark Denmark
8.Reference
 Haraguchi K, Sakamoto M, Matsuyama A, Yamamoto M, Dang T H, Nagasaka H, Uchida K, Ito Y, Kodamatani H, Horvat M, Chan H M, Rand M, Cirtiu C M, Kim B G, Nielsen F, Yamakawa A, Mashyanov N, Panichev N, Panova E, Watanabe T, Kaneko N, Yoshinaga J, Herwati R F, Suoth A E, Akagi H: Development of human hair reference material supporting the biomonitoring of methylmercury. Anal. Sci. 2020, 36, 561-567.
https://doi.org/10.2116/analsci.19SBP07
「NIMD-02 , 03 human urine (Low・High)」
1. Certified Value
 Certified values of NIMD-02 (Low) and NIMD-03 (High) shown below are traceable to the International System of Units (SI). Expanded uncertainties of certified values were obtained by multiplying standard uncertainties by a coverage factor k = 2.
NIMD-02 (Low)
Element Value
(µg/L)
Expanded Uncertainty
(µg/L)
Analytical Method
(refer to annotations below)
Total Mercury (THg) 0.292 0.057 1),2),3),4)
Cadmium (Cd) 0.154 0.030 4)
NIMD-03 (High)
Element Value
(µg/L)
Expanded Uncertainty
(µg/L)
Analytical Method
(refer to annotations below)
Total Mercury (THg) 4.80 0.56 1),2),3),4)
Analytical method
1) Thermal decomposition atomic absorption 
2) Cold vapor atomic absorption
3) Cold vapor atomic fluorescence
4) Inductively coupled plasma mass spectrometry  
2.Period of Validity
 The period of validity of NIMD-02 and NIMD-03 is 31 January 2022 if stored unopened and under the condition satisfying the instructions on storage shown below. The period of validity can be prolonged when further stability is ensured. Updated information on the period of validity will be announced here.
3.Product Form
 NIMD-02 and NIMD-03 are provided in a plastic tube. Four tubes are sealed as a set and each tube contains approximately 4 mL.
4.Instruction on Storage
 A short-term stability test for 4 weeks at 37 Celsius degree was conducted considering overseas shipment. A temperature indicator for 37 Celsius degree is enclosed with actual product. Immediately after receiving, store in a clean place at under – 20 degree Celsius and avoid light.
5.Instruction on Storage
 (1)Although NIMD-02 and NIMD-03 are sterilized with γ-ray irradiation, the potential risk as a source of diseases
    should be considered.
 (2)To homogenize the contents, shake the bottle well before use.
 (3)Ensure that the sample is at room temperature when measuring sample mass for analytical use.
6.Participants Involved in Characterization
No. Institution Country
1 Dong-A University Korea
2 IDEA Consultants, Inc. Japan
3 Institut national de santé publique du Québec Canada
4 Jožef Stefan Institute Slovenija
5 Kagoshima University Japan
6 Lumex Instruments Russia
7 Metropolitan Council Environmental Services US
8 National Institute for Minamata Disease (NIMD) Japan
9 Nippon Instruments Corporation (NIC) Japan
10 SHIMADZU Techno-Research, Inc. Japan
11 University of Ottawa Canada
12 University of Rochester School of Medicine and Dentistry US
13 Vietnam Academy of Science and Technology Vietnam
Institutions are in alphabetical order