| Takamitsu UNOKI, Ph.D. |
| Senior Researcher, Hygienic Chemistry Section, Department of Basic Medical Sciences
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| Research Subject |
Basic Research
FY 2020-2024
Research on the Methylmercury-Induced Alteration of Protein Function and its Protective Factors (Principal Investigator)
FY 2018-2019
Research on Selective Cytotoxicity of Methylmercury Focusing on Sulfur Nucleophiles (Principal Investigator)
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| Research Group |
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Pathomechanism Group
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| Publication |
[Original Article]
- Fujimura, M., Unoki, T. (2022). Preliminary evaluation of the mechanism underlying vulnerability/resistance to methylmercury toxicity by comparative gene expression profiling of rat primary cultured cerebrocortical and hippocampal neurons. J. Toxicol. Sci. 47, 211-21
DOI: 10.2131/jts.47.211
- Horai, S., Abiko, Y., Unoki, T., Shinkai, Y., Akiyama, M., Nakata, K., Kunisue, T., Kumagai, Y. (2022). Concentrations of nucleophilic sulfur species in small Indian mongoose (Herpestes auropunctatus) in Okinawa, Japan. Chemosphere 133833.
DOI: 10.1016/j.chemosphere.2022.133833
- Unoki, T., Akiyama, M., Shinkai, Y., Kumagai, Y., Fujimura, M. (2022). Spatio-temporal distribution of reactive sulfur species during methylmercury exposure in the rat brain. J. Toxicol. Sci. 47, 31-37.
DOI: 10.2131/jts.47.31
- Fujimura, M., Usuki, F., Unoki, T. (2020). Decreased plasma thiol antioxidant capacity precedes neurological signs in a rat methylmercury intoxication model. Food Chem. Toxicol. 146, 111810.
DOI: 10.1016/j.fct.2020.111810
- Akiyama, M., Unoki, T., Yoshida, E., Ding,Y., Yamakawa, H., Shinkai, Y., Ishii, I., Kumagai, Y. (2020). Repression of mercury accumulation and adverse effects of methylmercury exposure is mediated by cystathionine γ-lyase to produce reactive sulfur species in mouse brain. Toxicol. Lett. 330, 128-133.
DOI: 10.1016/j.toxlet.2020.05.007
- Akiyama, M., Unoki, T., Kumagai,Y. (2020). Combined exposure to environmental electrophiles enhances cytotoxicity and consumption of persulfide. Fundam. Toxicol. Sci. 7, 161-166.
DOI: 10.2131/fts.7.161
- Akiyama, M., Unoki, T., Shinkai, Y., Ishii, I., Ida, T., Akaike, T., Yamamoto, M., Kumagai, Y. (2019). Environmental electrophile-mediated toxicity in mice lacking Nrf2, CSE, or both. Environ. Health Perspect. 127, 67002.
DOI: 10.1289/EHP4949
- Akiyama, M., Shinkai, Y., Unoki, T., Shim, I., Ishii, I., and Kumagai, Y. (2017). The Capture of Cadmium by Reactive Polysulfides Attenuates Cadmium-Induced Adaptive Responses and Hepatotoxicity. Chem. Res. Toxicol. 30, 2209-2217.
DOI: 10.1021/acs.chemrestox.7b00278
- Abiko, Y., Shinkai, Y., Unoki, T., Hirose, R., Uehara, T., and Kumagai, Y. (2017). Polysulfide Na2S4 regulates the activation of PTEN/Akt/CREB signaling and cytotoxicity mediated by 1,4-naphthoquinone through formation of sulfur adducts. Sci. Rep. 7, 4814.
DOI: 10.1038/s41598-017-04590-z
- Abiko, Y., Sha, L., Shinkai, Y., Unoki, T., Luong, N.C., Tsuchiya, Y., Watanabe, Y., Hirose, R., Akaike, T., and Kumagai, Y. (2017). 1,4-Naphthoquinone activates the HSP90/HSF1 pathway through the S-arylation of HSP90 in A431 cells: Negative regulation of the redox signal transduction pathway by persulfides/polysulfides. Free Radic. Biol. Med. 104, 118-128.
DOI: 10.1016/j.freeradbiomed.2016.12.047
- Unoki, T., Abiko, Y., Toyama, T., Uehara, T., Tsuboi, K., Nishida, M., Kaji, T., and Kumagai, Y. (2016). Methylmercury, an environmental electrophile capable of activation and disruption of the Akt/CREB/Bcl-2 signal transduction pathway in SH-SY5Y cells. Sci. Rep. 6, 28944.
DOI: 10.1038/srep28944
- Hasegawa, H., Noguchi, J., Yamashita, M., Okada, R., Sugimoto, R., Furuya, M., Unoki, T., Funakoshi, Y., Baba, T., and Kanaho, Y. (2012). Phosphatidylinositol 4-phosphate 5-kinase is indispensable for mouse spermatogenesis. Biol. Reprod. 86, 136, 131-112.
DOI: 10.1095/biolreprod.110.089896
- Unoki, T., Matsuda, S., Kakegawa, W., Van, N.T., Kohda, K., Suzuki, A., Funakoshi, Y., Hasegawa, H., Yuzaki, M., and Kanaho, Y. (2012). NMDA receptor-mediated PIP5K activation to produce PI(4,5)P2 is essential for AMPA receptor endocytosis during LTD. Neuron 73, 135-148. F1000Prime Recommended
DOI: 10.1016/j.neuron.2011.09.034
- Nakano-Kobayashi, A., Yamazaki, M., Unoki, T., Hongu, T., Murata, C., Taguchi, R., Katada, T., Frohman, M.A., Yokozeki, T., and Kanaho, Y. (2007). Role of activation of PIP5Kγ661 by AP-2 complex in synaptic vesicle endocytosis. EMBO J. 26, 1105-1116.
DOI: 10.1038/sj.emboj.7601573
[Reviews]
- Unoki, T., Akiyama, M., Kumagai, Y. (2020). Nrf2 Activation and Its Coordination with the Protective Defense Systems in Response to Electrophilic Stress. Int. J. Mol. Sci. 21, 545.
DOI: 10.3390/ijms21020545
- Kumagai, Y., Akiyama, M., Unoki, T. (2019). Adaptive Responses to Electrophilic Stress and Reactive Sulfur Species as their Regulator Molecules. Toxicol. Res. 35, 303-310.
DOI: 10.5487/TR.2019.35.4.303
- Unoki, T., Akiyama, M., Kumagai, Y., Gonçalves, F.M., Farina, M., da Rocha, J.B.T., Aschner, M. (2018). Molecular pathways associated with methylmercury-induced Nrf2 modulation. Front. Genet. 9, 373.
DOI: 10.3389/fgene.2018.00373
- Kanaho, Y., and Unoki, T. (2012). Regulation and functions of the lipid kinase PIP5Kγ661 at synapses. Adv. Enzyme Regul. 52, 59-65.
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| Grant |
FY 2022-2024 Grant-in-Aid for Scientific Research (B), JSPS KAKENHI
Grant Number JP22H03768 (Co-Investigator)
FY 2021-2023 Grant-in-Aid for Scientific Research (C), JSPS KAKENHI
Grant Number JP21K06572 (Principal Investigator)
FY 2019-2022 Grant-in-Aid for Young Scientists, JSPS KAKENHI
Grant Number JP19K16368 (Principal Investigator)
FY 2016-2017 Grant-in-Aid for Young Scientists (B), JSPS KAKENHI
Grant Number JP16K18920 (Principal Investigator)
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| Resume |
2012 Ph.D. in Medical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba
2012-2014 Research Fellow, Faculty of Medicine, University of Tsukuba
2014-2018 Assistant Professor, Faculty of Medicine, University of Tsukuba
2018- Senior Researcher, Department of Basic Medical Sciences, National Institute for Minamata Disease
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